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Virologica Sinica, 19 (2) : 133-136, 2004
Research Article
Partial Cloning and Sequence Analysis of the gag Gene of Jaagsiekte Sheep Retrovirus NM Strain
1. College of Animal Science and Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China
2. College of life science, Northeast Agricultural University, Harbin 150030, China
In order to amplify partial gag gene of Jaagsiekte sheep retrovirus Inner Mongolia strain, a pair of primers was designed according to the GenBank sequence . The fragment of gag gene was obtained by polymerase chain reaction (PCR), and the gene was cloned into PMD-18 T vector and identified by EcoR I and Sal I digestion. The nucleotide and amino acid sequences of NM strain partial gag gene were compared with the counterpart sequeuces of South Africa strain (Accession No. NC-001494) and USA strain (Accession No. AF105220). The nucleotide and amino acid homology of partial gag gene were 83%, 81.5% and 84%, 83%, respectively. This is the first JSRV nucleotide sequence reported in China.
  Published online: 20 Apr 2004
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