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Virologica Sinica, 19 (2) : 146-148, 2004
Research Article
Regeneration of Transgenic Rice with the Rice Grassy Stunt Virus sp Gene
Institute of Plant Virology, Fujian Agricultural and Foresrtry University, Fuzhou 350002,China
Rice grassy stunt virus sp gene was transformed into rice by Agrobacterium mediated transformation. PCR analysis and Southern blot hybridization by using the genomic DNA of regenerated plants showed that the sp gene was transformed into rice. By using RT-PCR Southern blot hybridization, it was confirmed that the sp gene had been transtripted into mRNA.
  Published online: 20 Apr 2004
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