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Virologica Sinica, 19 (3) : 224-227, 2004
Research Article
Expression and Immunologenic Study of the Hantaan Virus Glycoprotein G1 and G2 by Using the Adenoviral Vector
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071 ,China
In order to study the immune responses of Hantaan virus envelope glycoproteins G1 and G2 in adenovirus expression system, G1 and G2 gene were amplified from Hantaan virus 76-118 and cloned to the plasmid pAdTrackCMV. Positive clones pAdTrackCMV-G1 and G2 were determined with restriction enzyme digestion. The recombinant adenovirus rAdeasy-G1 and rAdeasy-G2 were generated by bacterial homologous recombination with adenoviral plasmid pAdeasy-1 in E.coli BJ5183. After amplification in the 293 cell, the recombinant viruses were immuned to the Balb/c mice. The antibody reaction were detected by ELISA and Immunofluorescence Assay. Six rAdeasy-G1 immuned mice and four rAdeasy-G2 immuned mice produce antibody which can specifically react with the Hantaan virus. These results suggested a possible application of adenovir-
us expression system.
  Published online: 20 Jun 2004
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