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Virologica Sinica, 19 (4) : 315-319, 2004
Research Article
Selection of Differentially Expressed Signal Transduction-Associated Genes of Integrin in Hantavirus Infected Cells
Laboratory of Virology of Ministry of Education, Institute of Virology, Medical School, Wuhan University, Wuhan 430071, China
To develop the pathogenesis of Hantavirus infection, the differentially expressed signal trans- duction-associated genes of integrin in Hantavirus infected cells were investigated by using cDNA microarray. The primary human embryo pulmonary fibroblasts (HEPFs) were infected with Hantaan 76-118 virus. Total RNAs were extracted from infected and normal control group 6h, 24h and 96h post infection, respectively. During reverse transcription, the cDNAs were labeled by Cy5-dUTP and Cy3-dUTP. The labeled cDNAs hybridized with the Biostar-ⅡH gene chip. After hybridization, the fluorescence signal was analyzed by scanning. It showed that the expression level of most (113/150) Hantavirus receptor and signal transduction-associated genes was normal, however, 37 genes presented obvious change. Among them, expressions of 31 genes changed at 6h post infection. 13 genes were down-regulated, and 18 up-regulated. However, 5 and 4 genes expressed diversely at 24h and 96h post infection respectively. Furthermore, some integrin signal transduction pathway-associated genes such as the gene of some related protein kinases were up-regulated at 6h post infection. The gene of enhancer of filamentation 1 was down-regulated at 6h and 96h post infection. The gene of microtubule-associated protein 1A was up-regulated at 6h post infection, but down-regulated at 24h and 96h post infection. These indicate that Hantavirus infection is related to integrins.
  Published online: 25 Aug 2004
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