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Virologica Sinica, 19 (4) : 369-372, 2004
Research Article
Prokaryotic Expression of pp24 Gene of Md11 Strain of Marek’s Disease Virus
1. Animal Science and Technology College, Shandong Agricultural University, Shandong, Taian 271018, China
2. College of Veterinary Science, China Agricultural University, Beijing 100094, China
pp24 gene was amplified from genomic DNA of Marek’s disease viruses (MDV) Md11 strain by polymerase chain reaction(PCR) and then cloned into pGEX-6P-1 according to the right open reading frame(ORF). The recombinant plasmid was transformed into E.coli BL21 strain for expression with the induction of IPTG. SDS-PAGE and Westen-blotting with goat-anti-GST antibody validated the expression of GST-pp24 fusion protein. The expressed specific band was excised from the gel and injected into four-week-old mice 3 times, then the antiserum was collected from the mice and used for IFA with Chicken Embryonic Fibroblasts(CEF) infected by MDV Md11, CVI988 and GA strains respectively. The positive staining was found in the MDV plaques, which shows that the fusion protein of pp24 in vitro expressed has some epitopes of natural pp24.
  Published online: 25 Aug 2004
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