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Virologica Sinica, 19 (5) : 444-448, 2004
Research Article
Strutural Characterization and Phylogenetic Analysis of HIV-1 Genome of a Prevalent Subtype B′Strain in Central China
Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing, 100071, China
 Correspondence:
(277.66KB)  
Abstract
In order to analyze the gene characterization of Human immunodeficiency virus-1(HIV-1) prevalence in Henan Province, a HIV-1 strain, named CNHN24, was isolated from one HIV-1 infected blood donor. The proviral DNA was extracted from productively infected PBMCs (peripheral blood mononuclear cells) and full-length HIV-1 genomes were amplified. The sequences were determined by Walking sequencing. All of the reading frames were compared with HIV-1 RL42 and HIV-1 HXB2. The secondary structure prediction of the envelope proteins of HIV-1 CNHN24 and RL42 were performed by Anthewin software. Multiple alignments of the whole genome, env and V3 loop gene with domestic HIV-1 strains and standard representatives of HIV-1 were performed by Clustal software package, and phylogenetic tree were constructed. The results showed that the HIV-1 CNHN24 was B′subtype (according to V3 nucleotide sequence). There were more purines in the HIV-1 CNHN24 genome, it was 60.24%. Compared with HIV-1 RL42 and HXB2, the homegeneity of gag and pol reading frames was higher, env and non- structural genes was lower. The envelope proteins secondary structure of CNHN24 and RL4 were homoplastic, but the amino acid variability was higher in conservative C4. Phylogenetic trees showed the closest relationship between CNHN24 and RL42, the next was between HIV-1 Lai and HIV-1 HXB2. HIV-1 V3 sequence phylogenetic tree also indicated the closest relationship of CNHN24 to CR206 and RL42, which were isolated in Yunnan Province. We inferred that the HIV-1 CNHN24 may derive from Yunnan Province.
  Published online: 20 Oct 2004
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