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Virologica Sinica, 19 (5) : 454-457, 2004
Research Article
Sequence Clone and Analysis of Complete Genome of Porcine Circovirus (PCV2) from Piglets With PMWS
1.National Animal Quarantine Institute. Ministry of Agriculture, Qindao 266032, China
2. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
According to the published genomic sequence of Porcine circovirus(PCV2), a pair of specific primers was designed to amplify the complete genome of PCV2 from tissues of piglets with PMWS. Five complete genomes of PCV2, named Fujian PCV, Guangxi PCV, Hainan PCV, Hunan PCV and Shandong PCV, were amplified and cloned into pMD 18-T vector, respectively. The genomes of PCV2 isolates showed that four of them were 1767bp in length, one genome, FujianPCV was 1768bp. The five sequences were compared with other published genomes of PCVs by DNAstar. The results showed that the sequences of the five isolates were closely related to those of other PCV2 isolates in the Genbank, homology were 93% to 98.8%. However, there were only 68.4% to 70% nucleotide identity with the PCV1 isolates. The amino acid sequences of ORF1 and ORF2 of the isolates showed high identities, from 98.1% to 99.4% and 88% to 99.1%.
  Published online: 20 Oct 2004
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