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Virologica Sinica, 19 (5) : 462-466, 2004
Research Article
Expression of vp4 Gene from Porcine Rotavirus in E. coli
Molecular Virology Veterinary Medicine College, Northeast Agricultural University, Harbin 150030, China
A pair of primers was used in cloning and sequence analysis of vp4 gene from Porcine rotavirus JL94 isolated in China. According to the idea that 5’ segments of VP4 (1bp~750bp) principally control the activity of vp4 gene, a pair of another primers was used in cloning and obtained major antigen sites. The gene was cloned into the expression plasmid pGEX-6P-1. The recombinant plasmid VP4-pGEX-6P-1 was transformed into E. coli BL21(DE3)plays and induced with IPTG. Homology of amino acids between CRW-8 strain and BEN-307 strain is 96.98% and 98.05% respectively. The product of the VP4 gene was 26% of total bacterial protein of BL21. Western blot test and neutralization test circumstantiate the protein of expression has biological activity.
  Published online: 20 Oct 2004
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