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Virologica Sinica, 19 (6) : 559-562, 2004
Research Article
Humoral and Cellular Immunogenecity of DNA Vaccine of HIV-2 Chimeric Gene gag-gp105 in BALB/c
1. Key Laboratory of Genetic Engineering of PLA, Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130062, China
2. Institute of Vascular Medicine, Peking University Third Hospital, Beijing 100083, China
The recombinant expression plasmid of pVAX1gag-gp105 was constructed and transfected into BHK21 cells by lipofectamine, the expressed product was detected by indirect immunofluore scence. After the mice were immunized with pVAX1gag-gp105 and control plasmid, the number of CD4+and CD8+ subgroups of spleen T lymphocyte, the specific killing activities of spleen CTL and the titers of serum antibodies of the immunized mice were detected. Compared with vaccinated control groups, pVAX1gag-gp105 vaccinated group had significant differences in the titers of the serum antibodies (P<0.01), the numbers of spleen CD4+ and CD8+ T cells (P<0.05) and the specific killing activities of spleen CTL (P<0.01). These results manifested that the DNA vaccine based on HIV-2 chimeric gene gag-gp105 possessed good humoral and cellular immunogenecity in BALB/c mice.
Key Words: HIV-2;  gag-gp105;  DNA vaccine
  Published online: 20 Dec 2004
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