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Virologica Sinica, 19 (6) : 568-571, 2004
Research Article
Cloning and Sequence Analysis of vp2 Gene of Porcine Parvovirus SD-68 Strain
1.College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
2.China Animal Epizootiology Center, Qingdao 266032, China
3. Qilu Animal Health Products Factory, Jinan 250001, China
vp2 gene of Porcine parvovirus (PPV) SD-68 strain was cloned and sequenced .The results showed that vp2 gene of Porcine parvovirus (PPV) SD-68 strain was 1740bp and encoded a protein of 579 amino acids. vp2 gene of SD-68 strain exhibited over 99% nucleotide sequence identity and 96% amino acid sequence identity with other PPV strains: Kresse, SY-99, NADL-2(5075), NADL-2(4973), US-1. Phylogenetic tree analysis of vp2 gene showed the closest relationship between SD-68 strain and kresse strain. The difference between SD-68 strain and kresse strain was only on 378, 383 and 436 residues, which decided the tissue tropism of PPV, so the tissue tropism of SD-68 strain suggested more similar with kresse stain, that is to say, SD-68 was a dermatitis strain. Comparison of amino acid residues of virulent strain (kreese) and vaccine strain (NADL-2 and SD-68) suggested that 215, 378 and 383 were important residues that decided the pathogenicity of PPV.
  Published online: 20 Dec 2004
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