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Virologica Sinica, 19 (6) : 627-631, 2004
Research Article
cDNA Clone of Segment 8 and Prokaryotic Expression of Dendrolimus punctatus Wenshanensis cypovirus
1. Key Lab of Molecular Virology, Wuhan Institute of Virology, Chinese Academy of Sciences,Wuhan 430071,China
2. The Graduate School of Chinese Academy of Sciences, Bejing 100039, China
 Correspondence:
(869.90KB)  
Abstract
The nucleotide sequence of Dendrolimus punctatus Wenshanensis cypovirus genomic segment 8 (S8) has been determined. S8 consists 1332 nucleotides and encodes a putative protein of 390 amino acids with a molecular mass of approximately 44kDa (P44). Then, the fragment containing p44 gene was inserted into pET-28a expression vector and expressed in E.coli BL21. SDS-PAGE results showed that p44 gene has expressed.
  Published online: 20 Dec 2004
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