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Virologica Sinica, 20 (1) : 37-40, 2005
Research Article
Expression of Matrix and Capsid Protein of Bovine Immunodeficiency Virus
The matrix and the capsid are main structural proteins of Bovine immunodeficency virus (BIV) and play important roles in virus life cycle. In order to analyze these two proteins in vitro, we cloned these two genes into the prokaryotic expression vector pTXB and expressed in E. coli BL21(DE3) in a soluble form. After purification, the matrix without fusion peptide was obtained in milligram magnitude. The capsid was also expressed and purified in ten-milligram magnitude. We used the dissolvable BIV CA to immunized rabbit and got antiserum 53 d after first injection. Western blotting analysis showed that the antiserum could specifically recognize the native HIV CA protein. The protein could be used as the antigen to produce the antiserum and used in protein-protein interaction analysis.
Key Words: BIV;  Matrix;  Capsid;  pTXB system
  Published online: 20 Jan 2005
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