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Virologica Sinica, 20 (1) : 79-83, 2005
Research Article
Construction of a Recombinant HaSNPV Expressing GP64
There are two kinds of membrane fusion proteins encoded by baculovirus: GP64 and F protein. The membrane fusion protein of group I NPV is GP64, while group II NPV is F protein. In this paper. we tried to answer whether GP64 can be expressed and assembled into the BV of Group II NPV. By using Bac-to-Bac system, we successfully constructed recombinant baculoviruses HaSNPVgp64~+egfp~+ possessing GP64 of AcMNPV and EGFP, and HaSNPVegfp~+ with an EGFP as control. Western blotting analysis indicated that GP64 was expressed in HzAM1 cells infected with HaSNPVgp64~+egfp~+ and was packaged into progeny BVs.
  Published online: 20 Jan 2005
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