Hello guest, Register  |  Sign In
Virologica Sinica, 20 (3) : 243-246, 2005
Research Article
SDF-1 Gene Modulates Immune Responses Induced by HIV-1 Nucleic Acid Vaccine
 Correspondence:
(113.55KB)  
Abstract
To investigate the effect of SDF-1 gene immunization on immune response induced by HIV-1(Human immunodeficiency virus) nucleic acid vaccine and to explore new strategies for therapeutic HIV DNA vaccine. Balb/c mice were immunized with pCI-neoGAG alone or co-administered with the DNA encoding for SDF-1.Their sera were collected for analyzing anti-HIV antibody and IFN-γ by ELISA , and splenocytes were isolated for detecting antigen-specific lymphoproliferative responses and specific CTL response by MTT assay and LDH assay ,respectively. Our result showed that the anti-HIV antibody titers of mice co-immunized with pCI-neoGAG and the DNA encoding for SDF-1 were lower than that of mice immunized with pCI-neoGAG alone(P<0.01).The IFN-γ level of mice co-immunized with pCI-neoGAG and the DNA encoding for SDF-1was higher than that of mice immunized with pCI-neoGAG alone(P<0.01).Furthermore, compared with mice injected with pCI-neoGAG alone, the specific CTL cytotoxity activity and antigen-specific lymphoproliferative
Key Words: HIV-1;  DNA vaccination;  Immunization;  SDF-1
  Published online: 20 Jun 2005
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved