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Virologica Sinica, 20 (4) : 362-365, 2005
Research Article
Inhibition of Herpes Simplex Virus Infection by a GLP Isolated from Mycelium of Ganoderma lucidum
We reported here that a Ganoderma lucidum polysaccharide (GLP), one of the components extracted and purified from the liquid fermentation of mycelium of Ganoderma lucidum, was active against HSV-1infection in Vero cells. The CC_{50} (50% cytotoxic concentration) value of GLP for Vero cells growth was more than 2000μg/mL The EC_{50} (50% effective concentration) of GLP for virus yield reduction assay was 4.6μg/mL and 11μg/mL when virus or Vero cells were pre-mixed with GLP, 17μg/mL when virus and GLP were added into the cell culture simultaneously, and 50μg/mL when GLP was added after virus infection. Meanwhile, the selective index (SI, ratio of CC_{50} to EC_{50}) of GLP were more than 435, 182, 118 and 40, respectively. There was no significant antiviral activity to be detected when the GLP was presented in the culture after beginning infection and before progeny virus release. Quantitative real-time PCR of the infective supernatant further confirmed that the GLP blocked HSV-1 infection at early stages of th
  Published online: 20 Aug 2005
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