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Virologica Sinica, 20 (4) : 413-419, 2005
Research Article

Complete Genome Sequencing and Molecular Structure Prediction of C81 Attenuated Strain of

CSFV

 Correspondence:
(672.27KB)  
Abstract
Seven pair of primers covering the whole genome of Classical swine fever virus(CSFV) attenuated strain were devised according to the published sequences . 7 cDNA fragments were amplified by RT-PCR from PK-15 cell infected by CSFV attenuated strain. The amplifid fragments were further sequenced after cloned into the pMD18-T vector. The whole genome sequence of C81-strain was assembled by DNASIS (GenBank: AY663656). The genome is 12310 nucleotides in length and contains one large open reading frame encoding a polyprotein of 3898 amino acids. Sequence comparison with other CSFV strains revealed major differences in the open reading frame,84.4%~99.6% identities at the nucleotide level and 91.6%~99.4% identities at the amino acid level.26 ORF phylogenetic trees of CSFV strain were also protracted. The nucleotide sequence and secondary structure of CSFV 5′-untranslated region (5′-UTR) were compared and deduced, and found the major difference exists among diverse virus strains. Furthermore, functional domains and 3-D structure of CSFV/ C81 polyprotein were predicted.
  Published online: 20 Aug 2005
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