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Virologica Sinica, 20 (4) : 444-446, 2005
Research Article
Expression,Purification and Antigenicity Analysis of the HDV Antigen
Amplified HDV fragment by RT-PCR was cloned into T vector and PQE_{31} plasmid, which established a prokaryotic expression vector in E coli M_{15} The recombinant protein was purified by Ni-NTA column affinity chromatography, and identified by Western blot and ELISA: HDV antigen was expressed efficiently and had a well antigenicity character. It can be used in HDV clinical diagnosis and epidemiology survey.
  Published online: 20 Aug 2005
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