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Virologica Sinica, 20 (5) : 507-510, 2005
Prokaryotic Expression of the GP3 Protein of Porcine Reproductive and Respiratory Syndrome Virus S1 Strain
The recombinant plasmid pET-VP_2Ⅰ was transformed into BL21 competent cells and expressed in high level after induced with 1.0mmol/l IPTG.The expressed product was purified with His·Bind chromatograghy after being proved by Westen-blot,and was used as an antigen to establish indirect ELISA for detecting antibodies against PPV.The optional working circumstances for the ELISA(antigen concentration 3.5μg/mL;serum dilution 1∶40)were tried out with chess titration.The positive critertion of test serum of this ELISA is OD_490nm>0.51,the OD_490 ratio of the tested serum to the negative serum is higher than 2.1.
  Published online: 20 Oct 2005
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