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Virologica Sinica, 20 (5) : 511-514, 2005
Construction and Indentification of Recombinant Adenovirus Containing Multiple Antigen Epitopes of Swine Foot-and-Mouth Disease Virus
A recombinant replication defective Human adenovius serotype5 plasmid pAd2VP ,containing amino acids (21260)2(1412160)2(2002213) coding region of swine foot and mouth disease virus serotype O-VP1 , was const ructed using the method of homologous recombination in E.coli BJ5183. After the recombinant plasmid pAd2VP linearized with PacI t ransferred into HEK293A cell , the recombinant virus was isolated and purified in HEK2293A cells by t hree times plaque purification . This recombinant adenovirus could be stably passaged in HEK2293A cells and TCID50 was 10Key Words:
  Published online: 20 Oct 2005
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