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Virologica Sinica, 20 (5) : 519-521, 2005
Prokaryotic Expression of the GP3 Protein of Porcine Reproductive and Respiratory Syndrome Virus S1 Strain
The GP3 deleted hydrophobic N-terminal sequence(tGP3) of Porcine reproductive and respiratory syndrome virus(PRRSV) was cloned into prokaryotic expression vector pRSET.The recombinant protein(His)_6-GP3 was highly expressed in E.coli BL21 and could amount to 41% of the total proteins.It could be purified efficiently with affinity chromatography.Western-blotting analysis showed that the recombinant protein was able to react with PRRSV polyclonal antiserum.It can be used for the further investigation on immunogenicity and function of GP3 of PRRSV.
  Published online: 20 Oct 2005
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