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Virologica Sinica, 20 (5) : 530-533, 2005
Development of Real-time PCR for Detection of Porcine Reproductive and Respiratory Syndrome Virus
Primers and probe were designed from the nucleotide sequence within the ORF7 of Porcine reproductive and respiratory syndrome virus(PRRSV),and a quantitative TaqMan real-time PCR was developed for PRRSV detection.Specificity and sensitivity were compared with virus isolation and a conventional RT-PCR,and were found to be equal or superior to the reference methods.Reproducibility was tested and proved that the assay was reliable.Standard dilutions allowed absolute quantitation of the amount of viral RNA.The TaqMan real-time PCR described below is time-saving,easy to handle,exhibits a decreased risk of cross-contamination and is highly sensitive and specific.It is considered to be a powerful tool for the rapid detection of PRRSV.
Key Words: PRRSV;  GP3;  Prokaryotic expression
  Published online: 20 Oct 2005
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