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Virologica Sinica, 20 (6) : 582-585, 2005
Expression of the Gene Encoding the N Protein of SARS Virus in E.coli and Its Use in the Immunization of Horses
The gene encoding the N protein of SARS virus was cloned and expressed in E. coli. The re-combinant protein was identified by anti-SARS positive sera obtained from recovered SARS patients. The recombinant N protein (55 kDa) reacted specifically with anti-SARS positive sera. The recombinant N protein was purified and used to immunize horses, and the antibody was detected. The recombinant protein of SARS virus displayed specific reaction with SARS antibody. The results showed that the horses immunized with this recombinant protein produced higher antibody titers (1:2560). Our results provide information on the immunization against the SARS virus.
  Published online: 20 Dec 2005
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