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Virologica Sinica, 20 (6) : 590-593, 2005
Research Article
Expression and Purification of Nucleocaspid and Envelope Proteins of SARS Coronavirus
Genes encoding nucleocapsid (N) and envelope (E) proteins of SARS coronavirus (SARS-CoV) were obtained by RT-PCR and were cloned into the expression vector pGEX-KG. The genes were expressed in E. coli and the recombinant proteins were in a soluble form. The proteins were purified by affinity chromatography. The recombinant N protein reacted specifically with anti-SARS antibody. The results may provide a good tool for further research of immune responses and early diagnosis of SARS virus.
  Published online: 20 Dec 2005
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