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Virologica Sinica, 20 (6) : 623-625, 2005
Research Article
Comparion on Antigen Detection of Rabies Vaccine by Sandwich ELISA and NIH Assay
Sandwich ELISA with neutralizing McAbs against rabies virus glycoprotein can be used as a substitutive method to the NIH test. Kunming mice (12-14g) were inoculated ip with different dilution of rabies vaccines two times for NIH test (M-NIH), then challenged with CVS strain of rabies virus. At the same time, glycoprotein of rabies vaccines were tested with sandwich ELISA. A reference vaccine whose efficacy had been known was also tested with sandwich ELISA. Analysis of the data indicated that there was a significant positive correlation (0. 98) between E-NIHs and relevant M-NIHs of rabies vaccines. The range of E-NIHs resulted from one batch of rabies vaccine was between 2. 41 and 5. 85 while the range of M-NIH was between 5. 11 and 10. 19. There was a significant linear relation between E-NIHs and M-NIHs of rabies vaccines. E-NIHs showed a better reproducibility, lower cost and faster than M-NIHs. ELISA is a potential substitute of rabies vaccine NIH test.
Key Words: Rabies vaccine;  ELISA;  NIH assay
  Published online: 20 Dec 2005
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