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Virologica Sinica, 20 (6) : 647-651, 2005
Research Article
Amino Acid Sequence of a Chinese Isolate of Spring Viraemia Virus of Carp and Preliminary Analysis of the Glycoprotein Gene
Eight Spring Viraemia Viruses (SVCV) have been isolated from cultured carp and koi in China between 2002 to 2004. Based on the complete genome of SVCV reference strain, primers were designed and the glycoprotein genes from the 8 isolates were amplified, cloned and se-quenced. These genes were compared with other SVCVs in GenBank and showed nucleotide identities of more than 92%. There was more than 97. 7% nucleotide identities within the eight isolates. The homology was more than 94. 5% among the deduced amino acid sequences of the 8 isolates and 92. 0-94. 0% with other SVCV isolates. Phylogenetic analysis showed that the isolates shared evolutionary direction with USA isolates, and were different from other SVCV isolates from European countries. Nineteen common enzyme sites were found in the 8 isolates. Ten hy-drophobic regions, 10 possible antigenic sites and 10 transmembrane domains were also searched by the software and showed almost the same patterns. The functional sites include MYRISTYL, RGD, CK2-PHOSP
  Published online: 20 Dec 2005
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