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Virologica Sinica, 21 (1) : 16-20, 2006
Research Article
Action and Mechanism of Epstein Barr virus Latent Membrane Protein1 induced Immortalization of Mouse Embryonic Fibroblasts
Cancer Research Institute
Xiangya Medical College
Central South University
Changsha 410078
To investigate the action and mechanisms of Epstein Barr-virus(EBV) latent membrane protein1(LMP1) in stimulating the proliferation of primary mouse embryonic fibroblast (MEF) cells the retrovirus (RV) containing wild type LMP1(wt-LMP1) and mutant type LMP1(mt-LMP1);which replaced YYD with ID in tumor necrosis factor receptor associated death domain(TRADD)-binding site;were constructed and used to infect the MEF cells;respectively. Then cytokinetic and morphologic changes from infected cells at the course of passage were observed and found that control cells (MEF-LNSX) showed an apparent growth arrest from passages 8 (P8). In contrast;mt-LMP1 prolonged the cell (MEF-LMP1TRADD) doubling time since P10 and arrested growth at P19;while the MEF-LMP1 cell with wt-LMP1 had an accurate doubling time and displayed a immortalization. Meantime;the expression of cyclinA had a decrease in MEF-LNSX and MEF-LMP1TRADD after P4and P10 respectively. while in MEF-LMP1;cyclinA increased from P10 and kept at a high level. These results suggested that LMP1 can stimulate proliferation of MEF cells and induce their immortalization;while LMP1TRADD only induces an early proliferation of MEF cells. It is implied that TRADD domain might involve in LMP1-induced MEF cells immortalization.
  Published online: 20 Jan 2006
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