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Virologica Sinica, 21 (2) : 106-110, 2006
Research Article
Construction of a Novel Retargeted Adenoviral Vector with High Gene Transfer Efficiency into Hematopoietic Cells
Department of Pathophysiology, Anhui Medical University, Hefei 230032, China
2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China
The adenovirus subgenus C serotypes 5 (Ad5) vectors which have been used to transduce epithelial cells have very limited ability to infect hematopoietic cells. For feasible generation of fiber-retargeted adenoviral vectors, we have modified the versatile AdEasy system with a chimeric fiber gene encoding the Ad5 fiber tail domain and Ad11p fiber shaft and knob domains. An Ad5-based vector encoding the green fluorescent protein (GFP) gene under the control of the CMV promoter with Ad11p fiber receptor specificity was generated (Ad5F11p-GFP). The Ad5F11p-GFP vector-mediated gene transfer efficiency for some committed hematopoietic cell lines such as myeloblasts (K562) and monocytes (U937) was evaluated using flow cytometry and compared to that of Ad5-GFP, which also encodes the GFP gene under the control of the CMV promoter. The results showed that these cell lines were superiorly transduced by the Ad5F11p-GFP vector at the same MOI (multiplicity of infection, MOI) compared with the Ad5-GFP vector,more than 90% tested cells were infected by Ad5F11p-GFP adenovirus while less than 30% cells were infected by Ad5-GFP at 10 MOI.
  Published online: 20 Mar 2006
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