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Virologica Sinica, 21 (2) : 169-172, 2006
Research Article
Highly Soluble Expression of Carboxyl Terminus of Marek’s Disease Virus VP22 in E. coli
Key Lab of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China
Viral protein VP22 is indispensable for the growth and replication of Marek’s disease virus serotype 1(MDV-1). In this study, vp22 gene was amplified from and avirluent CVI988/Rispens strain. Antibodies specific to the carboxyl terminus of VP22 expressed in E. coli were generated by immunizing Balb/C mice 3 times. The antibodies reacted with the complete VP22 expressed in Chick Embryo Fibroblast (CEF) cells infected with CVI988 virus. It is interesting that the VP22 protein could be found in normal CEF cells around MDV plagues. The results suggested that VP22 may be transported among cwlls in MDV infection, which is vital to the studies on functions of VP22.
  Published online: 20 Mar 2006
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