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Virologica Sinica, 21 (3) : 231-234, 2006
Research Article
Construction of Recombinant Adenovirus Expressing Protective Antigen of Bacillus Anthracis and Analysis of Immuno-responses
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071,China
In this study, we constructed recombinant adenovirus containing protective antigen gene of Bacillus anthracis. The PA gene was obtained from pcDNA3.1-PA vector by PCR , and cloned into plasmid pAdTrack-CMV. The positive clone was linearized with PmeI restriction enzyme and transformed into E. coli BJ5183 cells with the adenoviral plasmid pAdEasy-1. Then vAd-PA was generated by homologous recombination. After linearized with PacI restriction enzyme, vAd-PA was transfected into HEK-293 cell line by lipofection. Expression of PA in 293 cells was confirmed by SDS-PAGE and Western-blot. The BALB/c mice were intramuscularly inoculated with the recombinant adenovirus and the specific antibody was detected by ELISA . The induced antibody titer was 2.8×103 (Geometric Mean).
  Published online: 20 May 2006
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