Hello guest, Register  |  Sign In
Virologica Sinica, 21 (3) : 257-260, 2006
Research Article
Construction of Recombinant Marek’s Disease Virus Vaccine Strain CVI988/Rispens Expressing the VP2 Protein of IBDV
Key Lab of Jiangsu preventive veterinary medicine, Yangzhou University, Yangzhou 225009, China
An expression cassette containing the VP2 gene of infectious bursal disease virus (IBDV) strain JS under the control of the CMV promoter and an enhancer in pcDNA3.1-VP2 was amplified by PCR and cloned into pUC18-US10 plasmid to yield the transferring vector pUC18-US10-VP2. The recombinant virus, designated rCVI988-VP2, was generated by co-transfection of CEF with pUC18-US10-VP2 and the Marek’s disease virus (MDV) vaccine CVI988/Rispens virus. PCR analysis and immunofluorenscence assays indicated that the recombinant virus was stable over 8 passages and that VP2 was expressed in CEF infected with the rCVI988-VP2. The data showed that the recombinant MDV expressing the VP2 gene of IBDV was successfully constructed and could prove to be a good strategy for IBD control.
  Published online: 20 May 2006
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved