Hello guest, Register  |  Sign In
Virologica Sinica, 21 (3) : 301-303, 2006
Brief Reports
Cloning and Phylogenetic Analyses of the Nucleoprotein Gene from Coronavirus of Pigeon Origin
School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 201101
The nucleoprotein (N) gene of the coronavirus of pigeon origin (PSH) was cloned and sequenced A pair of primers based on the nucleoprotein gene sequences of group 3 coronaviruses was designed and used to amplify the N gene of PSH The PCR product was purified and cloned into the pMD18-T vector. The positive recombinant pTN was used for sequencing. The cloned 1275bp fragment covered all the open reading frame of N gene and potentially encoded 409 amino acids. The results of comparative sequence analysis indicated that the nucleotide sequence of N gene of this strain shared 67.4%~96.6% identity with group 3 coronaviruses, and the predicted amino acid sequence shared 67.4%~97.8% identity, while only limited identity (<41.5%) was observed with group 1 and group 2 coronaviruses. Based on these results, PCoV PSH strain was identified as a member of group 3 coronaviruses.
  Published online: 20 May 2006
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved