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Virologica Sinica, 21 (4) : 324-327, 2006
Research Article
A New Reovirus Isolate: Biological Characteristics and sequencing of the S4 gene
State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing 100071,China
302 Hospital of PLA,Beijing 100039
The biological Characteristics and partial S4 gene sequence of a new Reovirus isolate was determined. Cell sensitivity testing on four cell lines was conducted by using the new isolate and the data showed that L929 cells and LLC-MK2 cells were highly permissive. The reovirus was resistant to ether, non-resistant to acid and heat in the physicochemical characteristics. It also had hemagglutination activity to human O-erythrocyte, and the titer reached up 1:32. Electron microscopic (EM) examination of LLC-MK2 cells infected with the reovirus revealed characteristic reovirus particles of about 70nm~80nm in diameter. Aggregates of reovirus particles showed in the cytoplasm of L929 cells. The putative S4 gene was amplified by PCR and the product had sequence homology to 3 prototypic strains T1L, T2J and T3D was 84%, 78%, 85%, respectively. All the results suggested that the new reovirus isolate is closely related to the known clusters of Reoviridae.
  Published online: 20 Jul 2006
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