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Virologica Sinica, 21 (4) : 339-342, 2006
Research Article
Hsp70-NP Recombinant Protein Enhances HTNV NP Specific Cellular Immune Response in vivo
1.Department of Pathology
2. Department of Orthopaedics
3. Department of Gastroenterology, Xi Jing hospital, the Fourth Military Medical University, Xi’an 710032, China
To examine whether the recombinant protein Hsp70-NP could induce the N-specific CTLs, we immunized C57/BL6 mice with purified protein Hsp70-NP, NP or Hsp70. The lymphoproliferative responses and CTL activities of spleen cells from vaccinated mice were determined. Accordingly, in order to get the target cells for cytotoxic assay, we tranfected pcDNA3.1/S into mice melanoma cells B16 with Lipofectamine2000, and selected stable transfectants with G418. RT-PCR, Western blots and immunofluorescence were used to identify the B16-N. The lymphoproliferative responses of spleen cells of mice vaccinated with Hsp70-NP, NP clearly showed proliferation in the presence of the N protein. But the stimulation indexes of splenocytes to NP of Hsp70-NP group were significantly higher than that of NP group. The CTL results showed that the cytotoxic effect of lymphocyte of the Hsp70-NP group was higher than that of the NP gorup. The results showed that Hsp70 could enhance the potency of NP to elicit HTNV NP specific cellular immune responses in vivo.
  Published online: 20 Jul 2006
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