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Virologica Sinica, 21 (4) : 380-384, 2006
Research Article
Construction and Identification of a Recombinant Canine Adenovirus Expressing Nucleocapsid Protein of Canine Distemper Virus
1.Institute of Military Veterinary, Academy of Military Medical Science of PLA, Changchun 130062, China
2.Department of Agricultural Science, Jilin University, Changchun 130062, China
 Correspondence:
(365.89KB)  
Abstract
The nucleocapsid protein gene (N) located 108 to 1679 situation of Canine distemper virus (CDV) genome and was immunogenicity protein with strong conservatism, so N gene was choosed as aim gene. A shuttle plasmid pVAXΔE3LPN containing the gene encoding the nucleocapsid protein of CDV was constructed by restriction endonuclease digestion and ligation. A recombinant plasmid pCAV-2-CDVLPN was constructed with pPoly2-CAV-2 vector containing CAV-2 SY genome. The plasmid pCAV-2-CDVLPN was transfected into MDCK cells by Lipofectamine and after three transfections, a typical CAV-2 cytopathic effect (CPE) was observed. We demonstrated that a recombinant canine adenovirus type-2 expressing nucleocapsid protein of canine distemper virus was successfully constructed and observed by electron microscopy, enzyme digestion, PCR and sequencing. The molecular mass of nucleocapsid protein was 58 kDa.
  Published online: 20 Jul 2006
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