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Virologica Sinica, 21 (4) : 401-404, 2006
Research Article
Expression and Functional Characterization of NS5 of Dendrolimus punctatus Cypovirus
1.Virus Molecular Ecology Lab, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2.The Graduate School of Chinese Academy of Sciences, Beijing 100039, China
The nucleotide sequence of segment 9 of Dendrolimus punctatus cypovirus (DpCPV) strain Hunan, which encodes the viral non-structural protein (NS5), was tagged with his-tag and expressed by pET32a (+). The recombinant protein was subsequently purified to homogeneity. By using the EMSA, which allows the detection of protein-RNA interactions in the test tube, the possible interactions were tested by the recombinant protein and the double-strand RNA (the genome of DpCPV).
  Published online: 20 Jul 2006
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