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Virologica Sinica, 21 (4) : 405-407, 2006
Brief Reports
Optimization of PCR for the Detection of Porcine Circovirus2
1.North-west Sci-Tech University of Agriculture and Forestry,Yangling 712100, China
2.National Animal Quarantine Institute of Ministry of Agriculture, Qingdao 266032, China
Three pairs of primers P1/P2, P3/P4 and P5/P6 of porcine circovirus 2 were designed and synthesized to compare the sensitivity and specialization to amplify products of 404bp, 647bp and 1773 bp. Optimum dilution of PK15 cell genome infected with PCV 2 was achieved with 10-6ng, 10-5ng to yield from the three pairs of primers. It was also confirmed that P1/P2 were 68.4% successful in detecting PCV2 in 57 swine samples from the province of Fujian, The other primers, P3/P4, P5/P6, were only 57.9% and 8.7% successful, respectively. We conclude that p1/p2 is the optimum primer pair for surveying incidents of PCV2 in pigs.
  Published online: 20 Jul 2006
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