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Virologica Sinica, 21 (4) : 408-410, 2006
Research Article
Antiserum Preparation against Rice Dwarf Virus Kunming Isolate and Detection by Immunocapture PCR Tests
1.The Biotechnology and Genetic Resources Institute,Yunnan Academy of Agricultural Sciences,Kunming 650223,China
2.College of Life Science, Peking University, Beijing 100871, China
By Using the modified method to extract Rice dwarf virus virions from infected rice leaves, a concentration 3.854mg/mL of purified virus virions reached, and the Rabbits was immunized with purified virions, yielding high titer antiserum; 1:20480 by the indirect enzyme-linked immunosorbent assay (I-ELISA) test. The antiserum successfully detected three rice samples from Kunming, Yuxi and Luliang by I-ELISA.Immunocapture polymerase chain reaction (IC-PCR), respectively was the utilized to show that serologic and molecular methods were successfully used for detection of Rice dwarf virus.
  Published online: 20 Jul 2006
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