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Virologica Sinica, 21 (5) : 413-416, 2006
Research Article
Expression and purification of HIV Integrase in E. col i and Application in HIV Diagnosis
1. Wuhan Institute of Virology,Chinese Academy of Sciences, Wuhan 430071, China
2. National Center for AIDS/STD Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 100050, China
3. Graduate University of Chinese Academy of Sciences, Beijing 100049, China
4. Hangzhou Ausia Bi-tech.Co.,Ltd, Hangzhou 310018, China
HIV-1 integrase P31 of RL42 strain, a B subtype isolate, was expressed in E.coli using pThioHisB vector(Invitrogen). The recombinant protein represented about 30% of total cellular proteins. and was purified by inclusion body washing and Ion Exchange Chromatography (IEX). The purity of P31 protein reached more than 95%. Purified P31 antigen displayed fairly good sensitivity and specificity when prepared into immunoblot strips and tested with HIV reference sera. This work established a foundation for the development of HIV detection kits.
Key Words: HIV-1;  Integrase;  P31;  Purification
  Published online: 20 Sep 2006
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