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Virologica Sinica, 21 (5) : 426-429, 2006
Research Article
In vitro Assays of an Antimicrobial Lipopeptide Produced by Bacillus subtilis fmbJ Against Pseudorabies Virus and Porcine Parvovirus
1.College of Food Science and Technology, Nanjing Agricultural University,Nanjing 210095, China
2. College of Food schence and Technology, Henan Agricultural University, Zheng zhou 450002, China
The effect and mechanism of antimicrobial lipopeptide (AMI) produced by a Bacillus subtilis fmbJ strain against Pseudorabies virus (PRV) and Porcine parvovirus (PPV) were studied. The Median Toxicosis Dose (TD50) and TD0 values of AMI on Porcine Kidney (PK-15) cells were 47.57mg/L and 18.9 mg/L, respectively. AMI strongly inhibited the cytopathic effect induced by PRV and PPV, and increase the survival rate of cell remarkably. However, it did not inhibit infection and multiplication of PPV and PRV in PK-15 cells. This study indicated that the antimicrobial lipopeptide could affect PRV and PPV directly but precluded infection of PK-15 cells. the effect of AMI in PK-15 cells was markedly lower than the antiviral drug Acyclovir (ACV). Since it had lower toxicity to PK-15 cell, we would study it further as an antiviral drug.
  Published online: 20 Sep 2006
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