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Virologica Sinica, 21 (5) : 449-453, 2006
Research Article
Application of an Improved Real-time TaqMan RT-PCR in FMDV Detection
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
In this study, an improved real-time RT-PCR was applied to quantification of Foot-and-mouth disease virus in vivo and in vitro. The results suggested that this method had a high sensitivity with up to less than 10 copies / reaction. Furthermore, this novel method was successfully used to quantify the transcriptional level of FMDV-3D gene in transfected BHK-21 cells and the resultant concentration of RNA level was up to 6.9×10 4 copies/μL. Compared with SYBR GreenⅠRT-PCR, six of eight samples testing the FMDV RNA copy number was up to 6.7-fold higher when determined by TaqMan RT-PCR, indicating that the improved method shows a high sensitivity in virus detection.
  Published online: 20 Sep 2006
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