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Virologica Sinica, 21 (5) : 454-457, 2006
Research Article
Expression of FMDV P1+2A Gene in BHK-21 Cells
Key Laboratory of Animal Virology, Ministry of Agriculture Lanzhou Veterinary Research Institute, Lanzhou 730046,China
In this study, recombinant expression plasmid pQE-Tri/P1+2A was constructed and then transfected into BHK-21 cell line mediated by liposome 2000. The results of SDS-PAGE、Western-blotting、fluorescence antibody and ELISA showed that FMDV P1+2A protein was expressed in BHK-21 cell line successfully and can be recognized by FMD positive serum. The result will lay a foundation for study of FMDV gene engineered vaccines as well as the development of diagnostic reagents.
  Published online: 20 Sep 2006
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