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Virologica Sinica, 21 (5) : 463-467, 2006
Research Article
Intracellular Localization of Pseudorabies Virus UL24 Protein
1. Key Laboratory of Animal Diseases Diagnosis and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
2. Department of Microbiology and Immunology, Nanjing Medical University, Nanjing 210029, China
A pair of primers were designed based on the PrV ul24 gene sequence in GenBank (NC006151). The UL24 protein coding sequence was amplified by PCR from PrV RongA strain genomic DNA. The product was cloned into pEGFP-N1 vector to generate the plasmid pUL24-GFP. Restriction endonuclease digestion, DNA sequencing and Western blots were employed to identify and authenticate pUL24-GFP. The ul24 DNA sequencing result was submitted to GenBank (DQ226544). Western blot analysis indicated that the UL24-GFP fusion protein was 45KD. After transfection of pUL24-GFP into eukaryotic cells, the intracellular localization of UL24-GFP fusion protein was examined by confocal microscopy and the result indicated that the fusion protein was localized mainly in nucleus.
Key Words: Pseudorabies virus;  PCR;  ul24;  GFP
  Published online: 20 Sep 2006
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