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Virologica Sinica, 21 (6) : 551-555, 2006
Research Article
Cloning and Characterization of the Polymerase Gene of Porcine Coronavirus
1. Key laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
2.College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
The polymerase gene, a non-structural gene from strain TS of transmissible gastroenteritis virus (TGEV), was amplified by RT-PCR primers designed based on the Purdue nucleotide sequence in GenBank. The expected 20054 bp product was obtained. The nucleotide sequence of ORF1 of TS shared nucleotide and amino acid identities of 98.8% and 99.0%, respectively with that of strain Pur46-MAD. The identity at the amino acid level for the ORF1 between TS and FIPV, PEDV, HCV299E,SARS was 87%、57%、57%、45%, respectively. RdRp was regarded to have an important role in replication and the results indicated that it was a conserved protein. The data also showed that there was a ribosomal slippage site UUUAAAC and three stem-loop structures in the ORF1a and ORF1b overlapping regions.
  Published online: 20 Nov 2006
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