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Virologica Sinica, 21 (6) : 599-603, 2006
Research Article
Inhibition of Hepatitis B Virus Replication by pRNA-escorted siRNA
1.Division of Clinical Immunology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
2. Department of Pathobiology and Purdue Cancer Research Center, Purdue University, West Lafayette, IN 47907, USA
The objective of our present study is to explore the potential use of pRNA as a bio-carrier of siRNA to inhibit HBV gene expression and replication. After co-transfected with pHA-HBs into 293T cells, HBVsi18-42, a pRNA-escorted siRNA, suppressed HBsAg and accumulated in the cells in a dose-dependent fashion. HBVsi18-42 substantially inhibited HBV gene expression and replication initiated by pHBV1.3 in HepG2 cells. In hydrodynamic injection mouse model, Balb/cJ mice were co-injected with pHBV1.3 and HBVsi18-42. Serum concentrations of HBsAg were analyzed by ELISA on days 1, 2, and 3 post-injection. HBV core protein in mouse liver was visualized by immunohistochemical staining. The results showed that the HBsAg levels in mice sera were reduced by 60%~90% in consecutive days relative to the control group, and the number of HBcAg positive hepatocytes in the mouse liver sections was decreased substantially by 79.1%. Our preliminary data showed that pRNA could be used as bio-carrier for the delivery of siRNA to knock down HBV gene expression and repress viral replication.
Key Words: RNAi;  pRNA;  Hepatitis B virus
  Published online: 20 Nov 2006
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