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Virologica Sinica, 22 (1) : 21-25, 2007
Research Article
Production And Characterization of Monoclonal Antibody of Shrimp White Syndrome Virus Envelope Protein VP28
1.State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2.Wuhan Institute of Biological Products, Ministry of Health, Wuhan 430060, China
3.Central China Normal University, College of Life Science, Wuhan 430079, China
The BALB/c mice were immunized with purified White Spot Syndrome Virus (WSSV). Six monoclonal antibody were selected, by ELISA, with VP28 protein expressed in E. Coli. The in vitro neutralization experiments showed that 4 of them could inhibit the virus infection to crayfish. Western-blot revealed that all these monoclonal antibodies were against the nature structure of VP28. The monoclonal antibody 7B4 was labeled with colloidal gold particles and used to locate the VP28 on virus envelope by immunogold labelling. These monoclonal antibodies could be used to develop immunological diagnosis methods of WSSV infection.
Key Words: WSSV;  Mab;  envelope;  localization;  neutralization
  Published online: 20 Feb 2007
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