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Virologica Sinica, 22 (1) : 61-67, 2007
Research Article
Development of lateral-flow immunoassay for WSSV with polyclonal antibodies raised against recombinant VP (19+28) fusion protein
State Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan, 430072, People’s Republic of China
We developed a sensitive and rapid lateral-flow immunoassay (LFIA) for WSSV, using a colloidal gold as an indicator. The fusion protein, VP (19+28), was expressed in E. coli, purified and used to prepare polyclonal antibodies. The purified anti-VP (19+28) IgG were conjugated with colloidal gold. Unconjugated anti-VP (19+28) IgG and goat anti-rabbit IgG were immobilized on nitrocellulose membranes. After assembly, three groups (5 individual animals in each group)of shrimp samples were tested which included healthy, moribund and dead shrimps. For each group, three different tissues (body juices, gills and hepatopancreas) were tested at the same time. In parallel, all the samples were also analyzed using an existing PCR for comparison. Out of 45 samples tested, 30 were detected as positive while 15 negative The results of LFIA correlate with those obtained by the PCR analysis, indicating that these two detection methods have the same efficacy in the limited number of samples tested in this preliminary study.
Key Words: Lateral-flow immunoassay;  WSSV;  VP19;  VP28
  Published online: 20 Feb 2007
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