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Virologica Sinica, 22 (1) : 74-79, 2007
Research Article
Comparison of three ELISA kits for the differentiation of Foot-and-Mouth disease virus-infected from vaccinated animals
Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, China
 Correspondence:
(1612.74KB)  
Abstract
A study was performed in order to validate FMDV 3ABC-I-ELISA kit developed in China for the differentiation of FMDV-infected animals from those had merely been vaccinated. Sets of sera from naive and vaccinated cattle as well as from cattle that had been infected were tested for antibodies against nonstructural proteins (NSPs) of FMDV by commercial diagnosis kits, Ceditest? FMDV-NS (Ceditest? kit), UBI? FMDV NONSTRUCTURAL PROTEIN ELISA DIRECTION INSERT (UBI? kit) and FMDV 3ABC-I-ELISA kit developed at the LanZhou Veterinary Research Institute. The test parameters (sensitivity and specificity) of the three kits were primarily determined, and the result obtained from domestic FMD 3ABC-I-ELISA kit was compared with that of obtained from two foreign kits respectively. The results indicate that the coincidence rate between FMD 3ABC-I-ELISA and Ceditest? kit is 98.05%, and the coincidence rate between FMD 3ABC-I-ELISA and UBI? kit is 94.4%; the sensitivity of both Ceditest? and FMD 3ABC-I-ELISA kit is 100%. However, the sensitivity of UBI? kit is only 81.8%. With sera from naive or vaccinated, but non-infected animals, the specificity of all tests exceeds 90%.
  Published online: 20 Feb 2007
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