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Virologica Sinica, 22 (4) : 257-265, 2007
Research Article
Phylogenetic Analysis of Orgyia pseudotsugata Single-nucleocapsid Nucleopolyhedrovirus
1. Laboratory of Virology, Wageningen University, Binnenhaven 11, Wageningen, 6709 PD, The Netherlands
2. Department of Biological Control and Quarantine, Institute of Plant Protection, Miczurina 20, Poznan, 60-318, Poland
3. Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, BC V8Z 1M5, Victoria, Canada
 Correspondence:
(191.23KB)  
Abstract
The Douglas-fir tussock moth Orgyia pseudotsugata (Lepidoptera: Lymantriidae) is a frequent defoliator of Douglas-fir and true firs in western USA and Canada. A single nucleopolyhedrovirus (SNPV) isolated from O. pseudotsugata larvae in Canada (OpSNPV) was previously analyzed via its polyhedrin gene, but is phylogenetic status was ambiguous. Sequences of four conserved baculovirus genes, polyhedrin, lef-8, pif-2 and dpol, were amplified from OpSNPV DNA in polymerase chain reactions using degenerate primer sets and their sequences were analyzed phylogenetically. The analysis revealed that OpSNPV belongs to group II NPVs and is most closely related to SNPVs that infect O. ericae and O. anartoides, respectively. These results show the need for multiple, concatenated gene phylogenies to classify baculoviruses.
  Published online: 20 Aug 2007
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