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Virologica Sinica, 22 (5) : 354-359, 2007
Research Article
Complete Genomic Sequence of a Chinese Isolate of Duck Hepatitis Virus
1. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. College of Animal Science and Technology, Anhui Agriculture University, Hefei 230036, China
 Correspondence:
(181.27KB)  
Abstract
The complete genomic sequence of Duck hepatitis virus 1 (DHV-1) ZJ-V isolate was sequenced and determined to be 7 691 nucleotides (nt) in length with a 5′-terminal un-translated region (UTR) of 626 nt and a 3′-terminal UTR of 315 nt (not including the poly(A) tail). One large open reading frame (ORF) was found within the genome (nt 627 to 7 373) coding for a polypeptide of 2 249 amino acids. Our data also showed that the poly (A) tail of DHV-1 has at least 22 A’s. Sequence comparison revealed significant homology (from 91.9% to 95.7%) between the protein sequences of the ZJ-V isolate and those of 21 reference isolates. Although DHV-1 has been classified as an unassigned virus in the Picornaviridae family, its genome showed some unique characteristics. DHV-1 contains 3 copies of the 2A gene and only 1 copy of the 3B gene, and its 3′-NCR is longer than those of other picornaviruses. Phylogenetic analysis to do sequence homology based on the VP1 protein sequences showed that the ZJ-V isolate shares high sequence homology with the reported DHV-1 isolates (from 92.9% to 99.2%), indicating that DHV-1 is genetically stable.
  Published online: 25 Oct 2007
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