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Virologica Sinica, 22 (5) : 360-365, 2007
Research Article
Immune Responses and Histopathological Changes in Rabbits Immunized with Inactivated SARS Coronavirus
1. Biomedicine Research & Development Center, Jinan University, Guangzhou 510630, China
2. Department of Chemistry and Biology, West Anhui University, Lu-an 237012, China
3. The First People’s Hospital, Shanghai Jiaotong University, Shanghai 200080, China
4. School of Public Health, Sun Yat-sen University, Guangzhou 510080, China
5. Center for Diseases Control and Prevention of Guangdong Province, Guangzhou 510300, Chin
 Correspondence:
(226.85KB)  
Abstract
To evaluate the immunogenicity of inactivated SARS coronavirus (SARS-CoV), three groups of rabbits were immunized three times at 2-week intervals with inactivated vaccine + adjuvant, adjuvant, and normal saline respectively. Eight batchs of serum were sampled from the auricular vein at day 7 to day 51, and specific IgG antibody titers and neutralizing antibody titers were detected by indirect ELISA and micro-cytopathic effect neutralizing test. Antibody specificity was identified by proteinchip assay. Histopathological changes were detected by H&E staining. The results showed that, rabbits in the experimental group immunized with inactivated SARS-CoV all generated specific IgG antibodies with neutralizing activity, which suggested the inactivated SARS-CoV could preserve its antigenicity well and elicit an effective humoral immune responses. The peak titer value of specific IgG antibody and neutralizing antibody reached 1:40960 and 1:2560 respectively. In the experimental group, no obvious histopathological changes was detected in the H&E stained slides of heart, spleen, kidney and testis samples, but the livers had slight histopathological changes, and the lungs presented remarkable histopathological changes. These findings are of importance for SARS-CoV inactivated vaccine development.
  Published online: 25 Oct 2007
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